Isolation and Identification of Bacteria Associated with Compost and Vermicompost of Wood Waste Substrates of Pycnanthus AngolensisfFor Cultivation Of Edible Mushroom Pleurotus Ostreatus.

Olabode, O. O.

Composting and vermicomposting process were carried out using wood dust of Pycnanthus angolensis with animal waste added as boosters for the cultivation of tropical mushroom Pleurotus ostreatus. Substrates were prepared with varying ratio of wood:animal waste and kept for a period of 10 weeks on shelf outdoor. The temperature reached a maximum of 42.5 in compost in 1 week of the process and dropped to a least of 24.5 in vermicompost in the same period. At the initial stage, more of gram positive bacteria were isolated and characterized, including Bacillus coagulans, Pediococcus cerevisae, Streptococcus faecalis. Lactobacillus subger, Micrococcus luteus, Actinomyces bovis, Actinomyces ericksonii. Some of the gram –ve bacteria isolated were Acinetobacter anitratis, Pseudomonas aeruginosa, Brucella melitenses. The highest total population of bacteria was 3.41x107 cfu/ml isolated from compost substrate of Pycnanthus angolensis wood drop and poultry dropping. The total bacteria population increased in the 1 week of process from 2.69x107 to 8.64 x 107 cfu/ml. In compost and 4.31 x 107 cfu/ml in vermicompost. Bacteria diversity and population reduced significantly at the end of the process. The most isolated genus is Bacillus sp.